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a b c The localization precision from Dhyana 95 was calculated to be 6.9 nm, an increase of 8% and 35% in localization precision compared to the 97%QE EMCCD camera (7.5 nm) and the 82%QE scmos camera (9.3 nm), respectively. It is 5 fps for STORM imaging and was taken over 4 frames to reconstruct the STORM images. For Epifluorescence images the exposure time is 1 ms giving imaging rate of 1 fps. Professor Ning Fang at Georgia State University STORM Imaging The beta tubulin of Hela cell was immunostained with mouse anti-tubulin primary antibody and Alexa fluor 647 conjugated Donkey anti mouse secondary antibody. We found Dhyana 95, the new scmos camera stood its own ground remarkably well and offered satisfactory performance across the board.
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On the other hand, Dhyana 95 had similar background fluorescence noise when comparing to the 82%QE scmos camera, which has 1.e-low read noise.ĥ Customer Case Studies and References Extensive tests and comparisons with other top-of-the-line EMCCD and scmos cameras have been performed in our laboratory. Tucsen Dhyana 95 The 97% QE EMCCD camera The 82% QE scmos camera -2 Under very weak laser illumination (<.2 kw cm ), Dhyana 95 camera showed comparable fluorescent intensity to the 97%QE EMCCD camera with 1 EM gain. Challenge the most extreme applications The Dhyana95's significant breakthrough in quantum efficiency means that scmos cameras have the potential to challenge much more extreme applications, with lower excitation energy, lower dye concentrations, and weaker photon signals, and still provide rich image information.
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2" Tucsen Dhyana "sCMOS Camera 2/3" EMCCD Camera 86dB ultra-high dynamic range The Dhyana95's 11μm pixel has a huge full well capacity, resulting in ultra-high dynamic range to support a variety of complex optical environments. 1 rsnr Test site: Wuhan National Laboratory for Optoelectronics -Huazhong University of Science and Technology Tucsen Dhyana 95(high gain) Tucsen Dhyana 95(low gain) The 97% QE EMCCD camera The 82% QE scmos camera Signal (photon) The Dhyana95 read noise is only 1.45 electrons(peak), therefore the signal to noise ratio is significantly better than other scmos cameras, and when the incident photons are>3, there is a better performance than EMCCD based cameras.Ĥ 2" large field of view The 2" array can not only adapt to more optical interfaces and deliver a greater field of view, but also results in fewer lens switches to find the area of interest on the sample. The higher the signal-to-noise ratio, the more obvious the recognizable signal is. Excellent signal to noise ratio Signal-to-noise ratio is the ratio of detectable signal to background noise. QExFF (%) Wavelength(nm) 95% Peak QE Tucsen Dhyana 95 The 97% QE EMCCD camera The 82% QE scmos camera The Dhyana95 uses backside-illuminated scmos thinned chip technology to avoid light interference from the wiring layer, thereby increasing the pixel area and improving the photoelectric conversion rate, as shown in the figure: at 56 nm, the quantum efficiency is 95%, which a front illuminated scmos camera cannot match, and is comparable to the best EMCCD camera. It is an important parameter to describe the photoelectric conversion capability of optoelectronic devices and is one of the important indicators to measure camera sensitivity. High Frame Speedģ Ultra-high quantum efficiency Quantum efficiency refers to the ratio of the average number of photoelectrons produced per unit time at a particular wavelength to the number of incident photons.
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11x11μ m Pixel BSI scmos sensor 1.45e-(Peak) Low Read Noise 9,e- Full Well Capacity -1 C Cooling Low Dark Current USB3. These innovative features open a new era to disciplines such as: life sciences spectral analysis astronomical observation and other cutting-edge research areas. 2 Backside-illuminated scmos technology Opening a new era of high sensitivity imaging applications! The Dhyana 95 is a highly sensitive scientific camera developed around back-illuminated scmos technology, not only with ultra-high quantum efficiency comparable to that of an EMCCD, but faster frame rate and excellent signal-to-noise performance, as well as a 2" field of view, 2-11nm spectral response and dynamic range of 11um pixels.